simoa hd x software Search Results


simoa hd 1 analyzer software  (Quanterix)
99
Quanterix simoa hd 1 analyzer software
Figure 1. Schematic of miRNA detection using <t>Simoa.</t> (A) Overview of the sandwich protocol. Capture probes were covalently coupled to microbeads, and then incubated with target miRNA and biotinylated detection probes to form a sandwich complex. The beads were washed and incubated with SG and RGP (enzyme and substrate) to produce a fluorescent product. (B) Detection was performed in the Simoa format as previously reported (32). As shown in the side view, following hybridization, microbeads suspended in fluorogenic substrate were loaded onto an array of femtoliter-size wells. After loading, the wells were sealed with oil, resulting in an array of isolated reaction chambers each of which contained either zero or one bead. If the enzyme-labeled complex was present on a bead, it generated a fluorescent product resulting in a detectable fluorescent signal. The array was imaged and analyzed to determine the total number of beads, and the number of ‘active’ wells was counted to calculate the average enzyme per bead (AEB). As shown in the top view, the number of active wells increased with increasing target concentration.
Simoa Hd 1 Analyzer Software, supplied by Quanterix, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/simoa+hd+x+software/HD-X+Automated+Immunoassay+Analyzer/pm28637221-88-34-38
Average 99 stars, based on 1 article reviews
simoa hd 1 analyzer software - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Schematic of miRNA detection using Simoa. (A) Overview of the sandwich protocol. Capture probes were covalently coupled to microbeads, and then incubated with target miRNA and biotinylated detection probes to form a sandwich complex. The beads were washed and incubated with SG and RGP (enzyme and substrate) to produce a fluorescent product. (B) Detection was performed in the Simoa format as previously reported (32). As shown in the side view, following hybridization, microbeads suspended in fluorogenic substrate were loaded onto an array of femtoliter-size wells. After loading, the wells were sealed with oil, resulting in an array of isolated reaction chambers each of which contained either zero or one bead. If the enzyme-labeled complex was present on a bead, it generated a fluorescent product resulting in a detectable fluorescent signal. The array was imaged and analyzed to determine the total number of beads, and the number of ‘active’ wells was counted to calculate the average enzyme per bead (AEB). As shown in the top view, the number of active wells increased with increasing target concentration.

Journal: Nucleic acids research

Article Title: Digital direct detection of microRNAs using single molecule arrays.

doi: 10.1093/nar/gkx542

Figure Lengend Snippet: Figure 1. Schematic of miRNA detection using Simoa. (A) Overview of the sandwich protocol. Capture probes were covalently coupled to microbeads, and then incubated with target miRNA and biotinylated detection probes to form a sandwich complex. The beads were washed and incubated with SG and RGP (enzyme and substrate) to produce a fluorescent product. (B) Detection was performed in the Simoa format as previously reported (32). As shown in the side view, following hybridization, microbeads suspended in fluorogenic substrate were loaded onto an array of femtoliter-size wells. After loading, the wells were sealed with oil, resulting in an array of isolated reaction chambers each of which contained either zero or one bead. If the enzyme-labeled complex was present on a bead, it generated a fluorescent product resulting in a detectable fluorescent signal. The array was imaged and analyzed to determine the total number of beads, and the number of ‘active’ wells was counted to calculate the average enzyme per bead (AEB). As shown in the top view, the number of active wells increased with increasing target concentration.

Article Snippet: To determine concentrations of miRNAs in these samples, a calibration curve for each target miRNA was fit to a 4PL nonlinear regression with a 1/y2 weighting factor, and unknown values were interpolated using the Simoa HD-1 Analyzer Software (Quanterix).

Techniques: Incubation, Hybridization, Isolation, Labeling, Generated, Concentration Assay

Figure 3. Three-plex Simoa assay for miR-21, miR-141 and miR-16, and cross reactivity profiles. (A) Multiplex assay for the simultaneous direct detection of three different miRNAs. (B–D) Cross-reactivity of the multiplex assay in the presence of (B) only miR-21, (C) only miR-141 and (D) only miR-16.

Journal: Nucleic acids research

Article Title: Digital direct detection of microRNAs using single molecule arrays.

doi: 10.1093/nar/gkx542

Figure Lengend Snippet: Figure 3. Three-plex Simoa assay for miR-21, miR-141 and miR-16, and cross reactivity profiles. (A) Multiplex assay for the simultaneous direct detection of three different miRNAs. (B–D) Cross-reactivity of the multiplex assay in the presence of (B) only miR-21, (C) only miR-141 and (D) only miR-16.

Article Snippet: To determine concentrations of miRNAs in these samples, a calibration curve for each target miRNA was fit to a 4PL nonlinear regression with a 1/y2 weighting factor, and unknown values were interpolated using the Simoa HD-1 Analyzer Software (Quanterix).

Techniques: Multiplex Assay

Figure 4. Three-plex Simoa assay for let-7a, let-7b and let-7c and cross-reactivity profiles. (A) Target, capture, and detection probe sequences used in this assay, and multiplex assay results for the direct detection of three different miRNAs simultaneously. (B–D) Cross-reactivity of the multiplex assay in the presence of (B) only let-7a, (C) only let-7b and (D) only let-7c. (E) Detection of let-7a, let-7b and let-7c in 12 samples using the three-plex Simoa assay. Actual spike-in concentrations for each of let-7a, let-7b and let-7c in the samples (S1–S12) are given in the table, and measured concentrations (after compensating for off-target signal as shown in Supplementary Figure S3 and Supplementary Table S3) are indicated in the plot.

Journal: Nucleic acids research

Article Title: Digital direct detection of microRNAs using single molecule arrays.

doi: 10.1093/nar/gkx542

Figure Lengend Snippet: Figure 4. Three-plex Simoa assay for let-7a, let-7b and let-7c and cross-reactivity profiles. (A) Target, capture, and detection probe sequences used in this assay, and multiplex assay results for the direct detection of three different miRNAs simultaneously. (B–D) Cross-reactivity of the multiplex assay in the presence of (B) only let-7a, (C) only let-7b and (D) only let-7c. (E) Detection of let-7a, let-7b and let-7c in 12 samples using the three-plex Simoa assay. Actual spike-in concentrations for each of let-7a, let-7b and let-7c in the samples (S1–S12) are given in the table, and measured concentrations (after compensating for off-target signal as shown in Supplementary Figure S3 and Supplementary Table S3) are indicated in the plot.

Article Snippet: To determine concentrations of miRNAs in these samples, a calibration curve for each target miRNA was fit to a 4PL nonlinear regression with a 1/y2 weighting factor, and unknown values were interpolated using the Simoa HD-1 Analyzer Software (Quanterix).

Techniques: Multiplex Assay

Figure 6. Direct detection of miR-21, miR-141 and miR-16 in a total RNA sample. Samples were serially diluted and concentrations were measured using the three-plex Simoa assay. (A) AEB values of the samples at varying amounts of total RNA. (B) Concentrations of miR-21, miR-141 and miR-16 in varying amounts of total RNA measured using RT-qPCR and the three-plex Simoa assay.

Journal: Nucleic acids research

Article Title: Digital direct detection of microRNAs using single molecule arrays.

doi: 10.1093/nar/gkx542

Figure Lengend Snippet: Figure 6. Direct detection of miR-21, miR-141 and miR-16 in a total RNA sample. Samples were serially diluted and concentrations were measured using the three-plex Simoa assay. (A) AEB values of the samples at varying amounts of total RNA. (B) Concentrations of miR-21, miR-141 and miR-16 in varying amounts of total RNA measured using RT-qPCR and the three-plex Simoa assay.

Article Snippet: To determine concentrations of miRNAs in these samples, a calibration curve for each target miRNA was fit to a 4PL nonlinear regression with a 1/y2 weighting factor, and unknown values were interpolated using the Simoa HD-1 Analyzer Software (Quanterix).

Techniques: Quantitative RT-PCR